abcam Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Cholesterol/ Cholesteryl Ester Assay Kit ab65359 provides a simple method for sensitive quantification of total cholesterol, free cholesterol, and cholesteryl esters, by colorimetric or fluorometric methods.

How the assay works

The majority of cholesterol in blood is in the form of cholesteryl esters. In the cholesterol assay protocol, these are hydrolyzed to cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to yield hydrogen peroxide. Hydrogen peroxide is then processed by an enzyme causing a reaction with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm).

The assay detects total cholesterol (cholesterol and cholesteryl esters) when cholesterol esterase is included in the reaction, or free cholesterol when it is not included. The amount of cholesteryl ester can be determined by subtracting the value of free cholesterol from the total (cholesterol plus cholesteryl esters).

Cholesterol assay protocol summary

• - Add samples and standards to wells.
• - Add total cholesterol reaction mix (with esterase) or free cholesterol reaction mix (without esterase) and incubate for 60 min at 37°C.
• - Analyze with microplate reader.

Related Cholesterol assay products

Cholesterol Assay Kit - HDL and LDL/VLDL Cholesterol Assay Kit - HDL and LDL/VLDLab65390 is identical to Cholesterol/ Cholesteryl Ester Assay Kit ab65359, except for the addition of a buffer and protocol for separation of HDL and LDL/VLDL fractions.

Related and recommended products

Other cholesterol assay kits include:

• - Cell-based Cholesterol assay kit Cholesterol Assay Kit (Cell-Based)ab133116
• - Cholesterol Efflux assay kit Cholesterol Efflux Assay Kit (Cell-based)ab196985
• - Cholesterol Uptake assay kit Cholesterol Uptake Assay Kitab236212

Learn more about our tools for obesity research, including antibodies and ELISA kits to adipogenesis and lipid metabolism markers, tools for GLP-1 receptor agonist research, and enzymatic assays to key biochemicals and enzymes involved in obesity-related metabolism.

Review our metabolism assays overview to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress here.

Other notes

This product is manufactured by BioVision, an Abcam company and was previously called K603 Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit. K603-100 is the same size as the 100 test size of ab65359.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye