ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) use a robust, simple ATP assay method. This kit can detect as low as 1 μM of ATP in various samples
How the assay works
ATP assay kit ab83355 uses the standard established assay protocol for fluorometric and colorimetric ATP determination kits:
- glycerol is phosphorylated by glycerol kinase in an ATP dependent reaction to produce glycerol 3-phosphate
- glycerol 3-phosphate is then acted on by glycerol phosphate oxidase to produce glycerone phosphate and hydrogen peroxide
- hydrogen peroxide is then processed by a peroxidase causing a reaction with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm).
ATP assay protocol summary
- - Add samples (deproteinized) and standards to wells
- - Add reaction mix and incubate for 30 min at room temp
- - Analyze with microplate reader
Related ATP assay products
If you require a more sensitive product, we recommend Luminescent ATP Detection Assay Kit (Luminescent ATP Detection Assay Kitab113849), which can detect as low as 1 pM of ATP.
How other researchers are using ATP Assay Kit ab83355
This ATP assay kit has been used in publications in a variety of sample types, including:
- - Human: cell culture lysates1, primary monocyte cell culture lysates2, HCT116 cell culture supernatants3
- - Mouse: heart tissue4, liver5, C2C12 and L929 cell lysates6, primary thymocyte cell culture lysates7, cardiac tissue8
- - Rat: primary hippocampal neuron cell culture lysates9, liver tissue10, skeletal muscle11
- - Pig: kidney cell culture lysates12, heart tissue13
- - C. elegans tissue14
- - Chlamydomonas reinhardtii algae15
References: 1 - Civallero M et al 2017, Na JY et al 2018, 2Gkirtzimanaki K et al 2018, 3Yang et al 2018; 4 - Singh SP et al 2018; 5 - Han SJ et al 2018; 6 - Alhindi Y et al 2019, Gregorczyk KP et al 2018; 7 - Simula L et al 2018; 8 - Litt MJ et al 2017, Samokhvalov V et al 2018; 9 - Zhao X et al 2018; 10 - Jing R et al 2018; 11 - Trinchese G et al 2018; 12 - Zou X et al 2018; 13 - Yuan F et al 2018; 14 - Pandey et al 2018; 15 - Ramanan R et al 2018
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K354 ATP Colorimetric/Fluorometric Assay Kit. K354-100 is the same size as the 100 test size of ab83355.
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