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ADP Assay Kit (Colorimetric/Fluorometric) (ab83359) provides
a convenient colorimetric and fluorometric method to measure ADP level. In the ADP assay, ADP is converted to ATP and pyruvate. The
generated pyruvate can be quantified by colorimetric (ODmax = 570 nm) or
fluorometric method (Ex/Em 535/587 nm). Conventionally, ADP levels are measured by
luciferase/luciferin mediated assays after ADP is converted to ATP. However,
the luciferase system is unstable and luminescence equipment is not generally
available in most laboratories. In comparison, this assay is simple, sensitive,
stable and high-throughput adaptable and can be used with conventional
microplate readers. The ADP assay can detect as low as 1 μM ADP in biological
samples.
This product is manufactured by BioVision, an Abcam company
and was previously called K355 ADP Colorimetric/Fluorometric Assay Kit.
K355-100 is the same size as the 100 test size of ab83359. ADP is a product of ATP de-phosphorylation and it can be
rephosphorylated to ATP. De-phosphorylation and re-phosphorylation occur via
various phosphatases, phosphorylases and kinases. ADP is stored in platelets
and can be released to interact with a variety of purinergic receptors. ADP
levels regulate several enzymes involved in intermediary metabolism. ADP
conversion to ATP primarily occurs within the mitochondrion and chloroplast
although several such processes occur in the cytoplasm. The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
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