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Iron Assay Kit ab83366 provides a simple convenient means of measuring ferrous (Fe2+) and/or ferric (Fe3+) iron in biological samples. How the assay works This ferrozine assay kit / iron assay kit uses an improved variant of the ferrozine molecule (ferene) which has been shown to give more accurate results for biological iron levels; that are closer to the results seen with mass spectrometry. In a ferrozine assay, free ferrous iron (Fe2+) reacts with the ferrozine molecule (in this case the ferene molecule) to produce a stable colored complex with absorbance that can be measured with a colorimetric plate reader. The ferene iron probe used in this assay kit has absorbance at 593 nm when complexed with ferrous iron (Fe2+). In Iron Assay Kit ab83366, in addition to the ferene iron probe and iron standard, an acidic assay buffer, with pH less than 5.5, is provided to release iron from iron carrier proteins. A reducing agent is also provided to convert ferric iron (Fe3+) to form Fe2+. Ferrous iron (Fe2+) can be measured alone, or by addition of the reducing agent the level of total iron (Fe2+ and Fe3+) can be measured. The level of ferric iron (Fe3+) is calculated by subtracting ferrous iron from total iron. A specific chelate chemical is included in the buffer to block copper ion (Cu2+) interference. The kit measures iron in the linear range of 0.4 to 10 nmol or 8 μM to 200 μM. Iron assay protocol summary - - Add samples and standards to wells
- - For Fe2+ assay add assay buffer only, for total iron (Fe2+ and Fe3+) add iron reducer
- - Incubate for 30 min at 37°C
- - Add Iron Probe and incubate for 60 min at 37°C
- - Analyze with microplate reader
Getting the best performance from ab83366 This assay is not suitable for use with samples containing EDTA or citrate. Related and recommended products Iron Assay Kit ab83366 is often used in combination with Lipid Peroxidation (MDA) Assay Kit Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)ab118970 and GSH/ GSSG Assay Kit GSH+GSSG / GSH Assay Kit (Colorimetric)ab239709 in the study of ferroptosis. To measure total iron binding capacity and iron levels in serum, we recommend Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kitab239715. For iron staining in tissues, we recommend Iron Stain Kit (Prussian Blue Stain) Iron Stain Kit (Prussian Blue Stain)ab150674. Other notes This product is manufactured by BioVision, an Abcam company and was previously called K390 Iron Colorimetric Assay Kit. K390-100 is the same size as the 100 test size of ab83366. The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section. |
