beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390) utilizes beta HB Dehydrogenase to generate a product which reacts with a colorimetric probe with an absorbance band at 450 nm.
The kit is an easy and accurate assay to measure beta HB levels in biological samples. The assay is linear for 1-20 nmol beta HB in up to 100 μL samples or 0.01-0.2 mM of beta HB samples.
beta Hydroxybutyrate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with microplate reader
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This product is manufactured by BioVision, an Abcam company and was previously called K632 β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit. K632-100 is the same size as the 100 test size of ab83390.
During diabetic ketoacidosis the liver produces ketone bodies (acetoacetate, acetone, and primarily beta-hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.
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