abcam Catalase Activity Assay Kit (Colorimetric/Fluorometric)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.

In the catalase activity assay protocol, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 535/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 μU of catalase activity.

Catalase activity assay protocol summary:

- add samples and standards to wells

- add stop solution into sample control wells and incubate at 25°C for 5 min

- add H2O2 solution into wells and incubate for 30 min at 25°C

- add stop solution

- add developer mix and incubate for 10 min at 25°C

- analyze with microplate reader

Technical Note: This assay is not recommended for use in urine samples due to the presense of reducing agents.

This product is manufactured by BioVision, an Abcam company and was previously called K773 Catalase Activity Colorimetric/Fluorometric Assay Kit. K773-100 is the same size as the 100 test size of ab83464.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye