biotechrabbit™ Taq DNA polymerase is a first-choice enzyme for all routine and molecular diagnostics PCR applications . The exceptional quality and purity of the enzyme ensures highest performance that is utilized by the diagnostics industry and research labs. The polymerase is suitable for standard and fast PCR applications giving high product yields from various templates with targets of up to 3 kb in size.
Taq DNA polymerase is a thermostable, highly processive 5'→3' DNA polymerase that has low 5'→ 3' exonuclease activity and lacks 3'→5' exonuclease (proofreading) activity. The latter allows incorporation of modified nucleotides.
The enzyme also exhibits deoxynucleotidyl transferase activity that results in the addition of extra A overhang at the 3'-ends of PCR products, allowing easy cloning of PCR products into vectors with T overhangs.
Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to check).
