biotechrabbit™ Pfu DNA Polymerase is a highly purified thermostable recombinant proofreading DNA polymerase. Pfu DNA Polymerase exhibits approximately 10 times higher accuracy than Taq DNA polymerase and amplifies targets up to 3–4 kb in size.
The enzyme catalyzes template-dependent nucleotide polymerization in the 5'→3' direction. Additionally the 3'→5' exonuclease (proofreading) activity corrects nucleotide incorporation errors, thereby increasing fidelity and accuracy of DNA polymerization. The enzyme has no 5'→3' exonuclease activity and no detectable reverse transcriptase activity and produces blunt-end PCR products.
For the most demanding applications, the supplied 5× PCR Enhancer can be optionally used for improving results when using templates with GC-rich sequences and complex structures.
