Efficient expression of up to 5 μg protein per reaction
Compatible with non-optimized, pro- and eukaryotic T7 promoter-based vectors as well as linear templates
Excellent homogeneity of large proteins (264 kDa expressed)
No endotoxins, allows direct use in cell-based assays
Upscaling using high yield CECF wheat germ kits
The biotechrabbit RTS 100 Wheat Germ Kit is a batch screening system allowing rapid protein expression within 2.5 hours. The kit allows use of a broad range of nonoptimized T7 promoter-based expression vectors from prokaryotic and eukaryotic origin in addition to established cell-free wheat germ vectors or linear templates. Using this approach, multiple constructs — including vectors that have not been optimized for wheat germ — can be screened rapidly for expression and functionality in parallel. The procedure saves precious time otherwise required for template generation. The new batch system yields up to 100 μg protein per ml reaction, which is sufficient for many downstream applications.
Fast parallel synthesis for screening purposes
Synthesis of mammalian or other proteins for functional testing without the disturbing endogenous protein background that is typical for in vivo expression
Synthesis of proteins dependent on eukaryotic chaperones
QPS-101T
THUNDERBIRD® Probe qPCR Mix 1 ml × 1
50x ROX reference dye 50 µl × 1
QPS-101
THUNDERBIRD® Probe qPCR Mix 1.67 ml × 3
50x ROX reference dye 250 µl × 1
QPS-101X5
THUNDERBIRD® Probe qPCR Mix (1.67 ml × 3) x 5
50x ROX reference dye 250 µl × 5
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
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