biological source:
synthetic (organic)
Quality Level: 300
Assay: ≥99%
Form: liquid
Concentration: 10 mM
Color: colorless
Solubility: H2O:
soluble at 20 °C
application(s): agriculture
foreign activity: DNase,
RNase, none detected
shipped in: dry
ice
storage temp.: −20°C
General description
Nucleotides are organic molecules that serve as the
monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks
of nucleic acids, nucleotides consist of a nitrogenous base (purine or
pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one
phosphate group. A nucleoside along with a phosphate group yields a nucleotide.
The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM
each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular
biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.
Application
dNTP Mix has been used:
- in
the PCR amplification of genomic DNA isolated from
insect,[1] fungi,[2] virus, human,[3]
- in
reverse transcription of total RNA to cDNA.[4][4]
- as a
component of the DNA amplification mixture for polymerase chain reaction
(PCR)[2][5][1]
- routine
and long PCR
- manual
and automated DNA sequencing
- cDNA
synthesis[6] and labeling reactions
Features and Benefits
- Purity
of each dNTP: Minimum 99%
- Conveniently
formulated; 1 μL is used per 50 μL PCR
- Equimolar
amounts of each dNTP means less pipetting
- Minimize
risk of contamination in PCR
- UltraPure
dNTPs can help maximize consistency and yields in critical PCR reactions
