Linear Formula: (CH3)2SO
CAS Number: 67-68-5
Molecular Weight: 78.13
Beilstein: 506008
EC Number: 200-664-3
MDL number: MFCD00002089
UNSPSC Code: 12191502
PubChem Substance ID:
24894214
NACRES: NA.52
grade: Molecular
Biology
technique(s):
DNA sequencing: suitable
PCR: suitable
transfection: suitable
bp: 189 °C
(lit.)
vapor pressure: 0.42
mmHg ( 20 °C)
Grade: Molecular
Biology
Quality Level: 200
vapor density: 2.7
(vs air)
vapor pressure: 0.42
mmHg ( 20 °C)
product line: BioReagent
Assay: ≥99.9%
Form: liquid
autoignition temp.:
573 °F
shelf life: Recommended
retest period - 2 years
expl. lim.: 42 %,
63 °F
technique(s):
DNA sequencing: suitable
PCR: suitable
transfection: suitable
Impurities:
≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)
Color: colorless
refractive index: n20/D
1.479 (lit.)
bp: 189 °C
(lit.)
mp: 16-19 °C
(lit.)
solubility: H2O:
miscible (completely)
density: 1.10 g/mL
(lit.)
absorption: passes
test
suitability: suitable
for molecular biology
foreign activity: DNase
and RNase, none detected
storage temp.: room
temp
SMILES string: CS(C)=O
InChI: 1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key: IAZDPXIOMUYVGZ-UHFFFAOYSA-N
General description
Features and Benefits:
- Suitable
for Molecular Applications
- Nuclease-Free
DMSO
- DNase-Free,
RNase-Free, Protease-Free, Phosphatase-Free
- Molecular
Biology Grade DMSO
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has
exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for
molecular biology applications. It has been analyzed for the presence of
nucleases and proteases.
Application
DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic
solvent with many applications in organic chemistry and molecular biology. DMSO
is routinely used in
- polymerase
chain reaction (PCR)
- amplification
of cDNA libraries
- DNA
sequencing
- column-loading
buffers for poly(A)+ RNA selection
- buffers
for the transformation of competent E. coli
- transfection
protocols.
Dimethyl sulfoxide has been used-
- as
an oligonucleotide solvent in array spotting[1]
- as a
solvent during miRNA microarray analysis and quantitative real-time PCR
(qRT-PCR)
- for
cell lysis during MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
- for
the preparation of the stock solution during MMP (mitochondrial membrane
potential)cytofluorimetric assessment
- in
the storage of human and animal cell lines and bacteriophage λ as a
cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is
recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are
not recommended.
Features and Benefits
This product is free from DNases, RNases, phosphatases and
proteases.
Caution
Supercools easily and remelts slowly at room temperature.
Solidified product can be re-liquified by warming to room temperature without
detriment to the product.
Other Notes
2024
CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research