Grade: Molecular
Biology
Quality Level: 200
Form: liquid
foreign activity: RNAse,
none detected
storage temp.: room
temp
General description
Gel loading buffer is used as a tracking dye during
electrophoresis. The dye has a slight negative charge and will migrate the same
direction as DNA, allowing the user to monitor the progress of molecules moving
through the gel. The rate of migration varies with gel composition. Dilute 1:3
to 1:6 with sample before loading.
Application
Gel Loading Buffer has been used for loading polymerase
chain reaction (PCR) amplified intergenic spacer region (ISR) sequence
samples,[1] PCR amplified DNA samples of the intestinal mucosa,[2] Trypanosoma
sp DNA[3] on agarose gel for electrophoresis.It is suitable for
use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE),
which may be part of Northern and Southern blot hybridization procedures.
Biochem/physiol Actions
Gel loading buffer is especially formulated for
non-denaturing polyacrylamide and agarose gel electrophoresis of nucleic acids.
The gel loading solution contains a bromophenol blue tracking dye and sucrose
to add density and facilitate sample loading. EDTA has been included to inhibit
nucleases that require divalent cations and SDS has been added to help
dissociate DNA-protein complexes which can interfere with electrophoresis.
Preparation Note
Gel loading buffer contains 0.05% bromophenol blue , 40%
sucrose, 0.1M EDTA (pH 8.0) and 0.5% SDS.
Other Notes
If crystals form, dissolve them by heating the solution to
65C for 10 minutes.
