Linear Formula: (CH3)2CHOH
CAS Number: 67-63-0
Molecular Weight: 60.10
Beilstein: 635639
EC Number: 200-661-7
MDL number: MFCD00011674
UNSPSC Code: 12352104
PubChem Substance ID:
24896158
NACRES: NA.52
Grade: Molecular
Biology
Assay: ≥99.5%
Technique(s):
DNA extraction: suitable
RNA extraction: suitable
Bp: 82 °C
(lit.)
Vapor pressure:
33 mmHg ( 20 °C)
44 mmHg ( 25 °C)
Product Name: 2-Propanol,
BioReagent, ≥99.5%, Molecular Biology
Grade: Molecular
Biology
Quality Level: 200
vapor density: 2.1
(vs air)
vapor pressure:
33 mmHg ( 20 °C)
44 mmHg ( 25 °C)
product line: BioReagent
Assay: ≥99.5%
Form: liquid
autoignition temp.:
750 °F
expl. lim.: 2.0-12.7 %,
93 °C
technique(s):
DNA extraction: suitable
RNA extraction: suitable
Impurities: ≤0.2%
water
water
Color: colorless
refractive index: n20/D
1.377 (lit.)
bp: 82 °C
(lit.)
mp: −89.5 °C
(lit.)
solubility: water:
soluble (completely)
density: 0.785 g/mL at
25 °C (lit.)
suitability: suitable
for precipitation of nucleic acids
storage temp.: room
temp
SMILES string: CC(C)O
InChI: 1S/C3H8O/c1-3(2)4/h3-4H,1-2H3
InChI key: KFZMGEQAYNKOFK-UHFFFAOYSA-N
General description
Isopropanol is a clear, colorless, polar organic solvent
commonly used in chemistry and molecular biology laboratories. It will dissolve
a wide range of chemicals, and evaporates quickly. This product (2-propanol) is
designated as molecular biology grade and is suitable for the precipitation of
nucleic acids.
Protocols for the use of isopropanol:
- DNA
precipitation from mammalian tissue, ethidium bromide extraction from DNA
samples, and RNA isolation from cells and tissues.[1]
- Protein
crystallization.[2][3][4]
- Isolate
retinoids from biological matrices for HPLC and HPLC-MS analysis.[5][6]
Application
2-Propanol has been used:
- in
DNA and RNA extraction[7][8][9][10]
- for
determination of hydroxyproline levels(The Merck Index, 12th ed)
- for
checking cell viability by MTT assay[11]
- in
microarray hybridization[12]
Suitable for use in DNA precipitation using standard
protocols. Also suitable as a solvent for making solutions for molecular
biology applications.
