Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in
measurement of cell proliferation. MTT produces a yellowish solution that is
converted to dark blue, water-insoluble MTT formazan by mitochondrial
dehydrogenases of living cells. The blue crystals are solubilized with
acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in
measurement of cell proliferation. MTT produces a yellowish solution that is
converted to dark blue, water-insoluble MTT formazan by mitochondrial
dehydrogenases of living cells. The blue crystals are solubilized with
acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
MTT has been used as a histochemical/cytochemical reagent and for the detection
of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to
binding of the cation by the cyanide trap used. MTT is rapidly reduced to the
formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate
has been used in oxidative systems.
Thiazolyl Blue Tetrazolium Bromide has been used as a
colorimetric metabolic activity indicator in cell viability assays (MTT
assay).[1][2][3][4] It has also been used to determine cell
proliferation.[5]
Other Notes
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