The Plant Genomic DNA Extraction Kit (Duo Buffer System)
provides a fast and simple method to isolate DNA from plant tissue and cells.
In the first step, the sample is lysed by homogenization. The lysate is treated
with RNase A to remove RNA. In the presence of a chaotropic salt, the genomic
DNA in the lysate binds to the glass fiber matrix in the spin column. The
contaminants are washed with an ethanol based wash buffer and purified genomic
DNA is eluted by low salt elution buffer or water. The protocol does not
require DNA phenol extraction and alcohol precipitation. The entire procedure
can be completed in 60 minutes.