The Viral Nucleic Acid Extraction Kit is specially designed
for purification of viral RNA/DNA from cell-free samples. The method utilises
detergents and a chaotropic salt to lyse the virus particles. Nucleic acid in
the chaotropic salt solution binds to the glass fiber matrix of the columns(1).
Carrier RNA enhances recovery of viral RNA in low-titer samples. Following
washing off of contaminants, the purified nucleic acid is eluted with
RNase-free water. The entire procedure can be completed in 20 minutes and the
purified nucleic acid is ready for RT-PCR and PCR.