General description
Amicon® Ultra-2 mL centrifugal devices with
Ultracel® 3,000 Dalton molecular weight cutoff filters provide
fast ultrafiltration, with the capability for high concentration factors and
easy concentrate recovery from dilute and complex sample matrices. For example,
Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically
retained at 100% .
The vertical design and available membrane surface area provide fast sample
processing, high sample recovery (typically greater than 90% of dilute starting
solution), and the capability for 50-fold concentration. Solute polarization
and subsequent fouling of the membrane are minimized by the vertical design,
and a physical deadstop in the filter device prevents spinning to dryness and
potential sample loss. Efficient recovery of the concentrated sample (retained
species) is achieved by a convenient reverse spin step after collecting the
filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2
mL devices are supplied non-sterile and are for single use only. With their
vertical membrane design and reverse spin, Amicon® Ultra 2 mL
filters deliver the highest recovery with shortest spin times.
We are committed to bringing you Greener Alternative
Products, which have been re-engineered to enable more sustainable research
solutions. The inner filtration device is made from bio-circular material
allocated using the ISCC mass balance approach. Click here for
more information.
Application
- Ultrafiltration
with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration
of biological samples containing antigens, antibodies, enzymes, nucleic
acids, or microorganisms.
- Purification
of macromolecular components found in tissue culture extracts or cell
lysates.
- Primer
removal, PCR cleanup, removal of linkers or macromolecular labels from a
reaction mix, and protein removal prior to HPLC.
- Desalting,
buffer exchange and protein dialysis.
- Size
exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated
cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater
than 90%
- Concentrates
2 mL down to 15-70 μL
- 98%
sample recovery
- Fast
processing time: 10 to 30-minute spin times
- Engineered
dead stop
- Reverse
spin capability provides consistent recoveries
Linkage
Replaces: 4202
Physical form
Color Code: Clear
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt,
Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt,
Germany
