General description
Amicon® Ultra-2 centrifugal filter devices
offer fast ultrafiltration. It has the potential to recover high concentration
factors and easy concentrate from dilute and complex sample matrices. Amicon® Ultra-2
devices are supplied non-sterile and are for single use only.
We are committed to bringing you Greener Alternative
Products, which have been re-engineered to enable more sustainable research
solutions. The inner filtration device is made from bio-circular material
allocated using the ISCC mass balance approach. Click here for
more information.
Application
- Ultrafiltration
with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration
of biological samples containing antigens, antibodies, enzymes, nucleic
acids, or microorganisms.
- Purification
of macromolecular components found in tissue culture extracts or cell
lysates.
- Primer
removal, PCR cleanup, removal of linkers or macromolecular labels from a
reaction mix, and protein removal prior to HPLC.
- Desalting,
buffer exchange and protein dialysis.
- Size
exclusion and protein fractionation.
Amicon® Ultra-2 Centrifugal Filter Unit has
been used:
- to
reduce large volume biofluids prior to extracellular vesicle (EV)
isolation or to concentrate EVs[1]
- Concentration
of biological samples containing antigens, antibodies, enzymes, nucleic
acids (DNA/RNA samples, either single- or double-stranded),
microorganisms, column eluates, and purified samples
- Purification
of macromolecular components found in tissue culture extracts and cell
lysates, removal of primer, linkers, or molecular labels from a reaction
mix, and protein removal before high-performance liquid chromatography
(HPLC)
- Desalting,
buffer exchange[2], or diafiltration
Features and Benefits
- Amicon® Ultra
2ml 10K 24PK
- Concentrates
2 mL down to 15-70 μl
- 98%
sample recovery
- Fast
processing time - 10 to 30 minute spin times
- Engineered
dead stop
- The
reverse spin capability provides consistent recoveries
- Ultracel® regenerated
cellulose membrane with 10 kDa NMWCO delivers retentate recovery of
greater than 90%
- Concentrates
2 mL down to 15-70 μL
- 98%
sample recovery
- Fast
processing time: 10 to 30-minute spin times
- Engineered
dead stop
- Reverse
spin capability provides consistent recoveries
Linkage
Replaces: 4205
Other Notes
The Amicon® Ultra-2 device is supplied with
two tubes. During operation, one tube is used to collect filtrate; the other to
cap the device during concentration and subsequently to recover the
concentrated sample. Amicon Ultra-2 Centrifugal filter devices are for research
use only and not for use in diagnostic procedures.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt,
Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt,
Germany
