General description
Amicon® Ultra-2 mL centrifugal devices with
Ultracel® 100,000 Dalton molecular weight cutoff filters
provide fast ultrafiltration, with the capability for high concentration
factors and easy concentrate recovery from dilute and complex sample matrices.
For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically
retained at 95% .
The vertical design and available membrane surface area provide fast sample
processing, high sample recovery (typically greater than 90% of dilute starting
solution), and the capability for 50-fold concentration. Solute polarization
and subsequent fouling of the membrane are minimized by the vertical design,
and a physical deadstop in the filter device prevents spinning to dryness and
potential sample loss. Efficient recovery of the concentrated sample (retained
species) is achieved by a convenient reverse spin step after collecting the
filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2
mL devices are supplied non-sterile and are for single use only. With their
vertical membrane design and reverse spin, Amicon® Ultra 2 mL
filters deliver the highest recovery with shortest spin times.
We are committed to bringing you Greener Alternative
Products, which have been re-engineered to enable more sustainable research
solutions. The inner filtration device is made from bio-circular material
allocated using the ISCC mass balance approach. Click here for
more information.
Application
- Ultrafiltration
with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration
of biological samples containing antigens, antibodies, enzymes, nucleic
acids, or microorganisms.
- Purification
of macromolecular components found in tissue culture extracts or cell
lysates.
- Primer
removal, PCR cleanup, removal of linkers or macromolecular labels from a
reaction mix, and protein removal prior to HPLC.
- Desalting,
buffer exchange and protein dialysis.
- Size
exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated
cellulose membrane with 100 kDa NMWCO delivers retentate recovery of
greater than 90%
- Concentrates
2 mL down to 15-70 μL
- 98%
sample recovery
- Fast
processing time: 10 to 30-minute spin times
- Engineered
dead stop
- Reverse
spin capability provides consistent recoveries
Linkage
Replaces: 4211
Physical form
Color Code: Clear
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt,
Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt,
Germany
