General description
Amicon® Ultra-0.5 centrifugal filter devices
provide fast ultrafiltration, with the capability for high concentration
factors and easy concentrate recovery from dilute and complex sample matrices.
For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is
typically retained at >95%.
The vertical design and available membrane surface area provide fast sample
processing, high sample recovery (typically greater than 90% of dilute starting
solution), and the capability for 30-fold concentration. Solute polarization
and subsequent fouling of the membrane are minimized by the vertical design,
and a physical deadstop in the filter device prevents spinning to dryness and
potential sample loss. Efficient recovery of the concentrated sample (retained
species) is achieved by a convenient reverse spin step after collecting the
filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for
single use only. With their vertical membrane design and reverse spin, Amicon® Ultra
0.5 mL filters deliver great performance and lower spin times.
We are committed to bringing you Greener Alternative
Products, which have been re-engineered to enable more sustainable research
solutions. The inner filtration device is made from bio-circular material
allocated using the ISCC mass balance approach. Click here for
more information.
Application
- Ultrafiltration
with 3,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration
of biological samples containing antigens, antibodies, enzymes, nucleic
acids, or microorganisms
- Purification
of macromolecular components found in tissue culture extracts or cell
lysates, primer removal, linkers, or macromolecular labels from a reaction
mix, and protein removal prior to HPLC
- Desalting,
buffer exchange, and protein dialysis
Features and Benefits
- Ultracel® regenerated
cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater
than 90%
- Concentrates
500 μL down to 15 μL
- Concentration
factor 25x to 30x
- High
sample recovery (greater than 90% of dilute starting solution)
- Fast
processing time: 10 to 30 minutes
- Reverse
spin capability provides consistent recoveries
Physical form
Color Code: Clear
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt,
Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt,
Germany
