General description
Amicon® Ultra 4 mL centrifugal devices with
Ultracel® 100,000 Dalton molecular weight cutoff filters
provide fast ultrafiltration, and reliable retention of macromolecules. For
example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >95%
.
The vertical design and available membrane surface area provide fast sample
processing, high sample recovery (typically greater than 90% of dilute starting
solution), and the capability for 80-fold concentration. Solute polarization
and subsequent fouling of the membrane are minimized by the vertical design,
and a physical deadstop in the filter device prevents spinning to dryness and
potential sample loss.
The concentrate is collected from the filter device sample reservoir using a
pipetter, while the ultrafiltrate is collected in the provided centrifuge tube.
The device can be spun in a swinging bucket or fixed angle rotor. With their
vertical membrane design and deadstop, Amicon® Ultra 15 mL
filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative
Products, which have been re-engineered to enable more sustainable research
solutions. The inner filtration device is made from 60% bio-circular material
allocated using the ISCC mass balance approach. Click here for
more information.
Application
- Ultrafiltration
with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration
of biological samples containing antigens, antibodies, enzymes, nucleic
acids, or microorganisms.
- Purification
of macromolecular components found in tissue culture extracts or cell
lysates and protein removal prior to HPLC.
- Concentration
of dilute or pre-purified proteins from column eluents.
- Desalting,
buffer exchange and protein dialysis.
- Size
exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated
cellulose membrane with 100 kDa NMWCO delivers retentate recovery of
greater than 90%.
- Vertical
membrane reduces concentration polarization for ultra-fast spin times (as
fast as 5 minutes).
- Heat-sealed
membrane minimizes downstream extractables.
- 100%
integrity tested for reliable performance.
- Convenient
sample monitoring with translucent housing and volume gradations.
- Direct
pipette sample access eliminates processing step to recover concentrate.
- High
concentration factors of 80–100X.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt,
Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt,
Germany
