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Quantabio qScript XLT cDNA SuperMix

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Cat No.
MT-MC-95161-025 Size: 25 x 20-µL reactions (1 x 100 µL)
MT-MC-95161-100 Size: 100 x 20-µL reactions (1 x 400 µL)
MT-MC-95161-500 Size: 500 x 20-µL reactions (2 x 1 mL)
qScript XLT cDNA SuperMix Premium Performance with SuperMix Benefits



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  • Stabilized 5X concentrated master mix simplifies reaction assembly and improves accuracy by minimizing pipetting steps
  • Optimized, double pre-primed SuperMix provides equal representation of 5′ and 3′ RNA sequences ≤ 1kb for quantitative and conventional two-step RT-PCR
  • Improved RNA template binding affinity for increased yields with problematic sequences or complex secondary structure
  • Perform more qPCR assays from limiting amounts of RNA with 2-3 fold improved cDNA yields over older enzyme technologies
  • Broad linear dynamic detection range spanning up to 108 fold



qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase mutant with reduced RNase H activity and improved yield and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than our previous qScript cDNA SuperMix cDNA synthesis kits, which utilize an engineered RNase H(+) reverse transcriptase mutant.


Contents

Single-tube, 5X concentrated reagent containing:

  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
  • Recombinant ribonuclease inhibitor protein (RIP)
  • qScript XLT reverse transcriptase
  • Titrated concentrations of random hexamer and oligo(dT) primer
  • Proprietary enzyme stabilizers and performance-enhancing additives
  •  QPS-101T
    • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
    • 50x ROX reference dye    50 µl × 1
  •  
  • QPS-101
    • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
    • 50x ROX reference dye    250 µl × 1
  •  
  • QPS-101X5
    • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
    • 50x ROX reference dye    250 µl × 5




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye