The Plant Genomic DNA Extraction Kit (Duo Buffer System)
provides a fast and simple method to isolate DNA from plant tissue and cells.
In the first step, the sample is lysed by homogenization. The lysate is treated
with RNase A to remove RNA. In the presence of a chaotropic salt, the genomic
DNA in the lysate binds to the glass fiber matrix in the spin column. The
contaminants are washed with an ethanol based wash buffer and purified genomic
DNA is eluted by low salt elution buffer or water. The protocol does not
require DNA phenol extraction and alcohol precipitation. The entire procedure
can be completed in 60 minutes.
The quality of Genomic DNA Extraction Mini Kit (Tissue) is
tested on a lot-to-lot basis. The kits are tested by isolation of genomic DNA
from 10mg of mouse liver. Purified DNA is quantified with a spectrophotometer.
Genomic DNA yield is more than 10μg with A260/A280 ratio 1.7 to 1.9.
GP1 Buffer x 50ml GPX1 Buffer x 50ml GP2 Buffer x 15ml GP3
Buffer x 30ml W1 Buffer x 50ml Wash Buffer x 25ml
Elution Buffer x 30ml RNase A (10mg/ml) x 550ul Filter Column x
100pcs
GD Column x 100pcs Collection Tube x 200pcs
The quality of HiYield™ Plasmid Mini Kit is test on a
lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml
culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml).
More than 20μg of plasmid DNA was quantified with spectrophotometer.
QPS-101T
THUNDERBIRD® Probe qPCR Mix 1 ml × 1
50x ROX reference dye 50 µl × 1
QPS-101
THUNDERBIRD® Probe qPCR Mix 1.67 ml × 3
50x ROX reference dye 250 µl × 1
QPS-101X5
THUNDERBIRD® Probe qPCR Mix (1.67 ml × 3) x 5
50x ROX reference dye 250 µl × 5
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
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