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RBCBioscience HiYield Gel/PCR DNA Fragments Extraction Kit 300 preps

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MT-MC-YDF300

 
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The HiYield ™ Gel / PCR DNA Fragments Extraction Kit is designed to recover or concentrate DNA fragments (50bp - 10Kb) from agarose gels, PCR or other enzymatic reactions. The unique dual purpose application and high yield DNA / minicolumn make this kit exceptional value. The method uses a chaotropic salt , guanidine thiocyanate to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column. Following washing off contaminants, the purified DNA fragments are eluted by addition of low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation .

The quality of HiYield™ Gel / PCR DNA Fragments Extraction Kit is tested on a lot - to - lot basis . The efficiency of DNA recovery is tested by isolation of DNA fragments of various sizes from either aqueous solution or agarose gel . The purified DNA is checked by agarose gel analysis.

DF Buffer x 200ml 
Wash Buffer x 40ml 
Elution Buffer x 30ml 
DF Column x 300pcs
Collection Tube x 300pcs

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

  •  QPS-101T
    • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
    • 50x ROX reference dye    50 µl × 1
  •  
  • QPS-101
    • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
    • 50x ROX reference dye    250 µl × 1
  •  
  • QPS-101X5
    • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
    • 50x ROX reference dye    250 µl × 5




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye