RBCBioscience Total RNA Extraction Mini Kit (Plant) 100 preps

The Plant Total RNA Mini Kit provides a fast and simple method to isolate total RNA from plant tissue and cells. In this process the sample is first ground in liquid nitrogen and filtrated by filter column to remove cell debris. In the presence of binding buffer and a chaotropic salt the total RNA in the lysate binds to the glass fiber matrix in the spin column. The optional DNase treatments can remove DNA residues and contaminants are washed out with an ethanol based wash buffer. Finally, the purified total RNA is eluted by RNase - free water. The protocol does not require phenol extraction and Ethanol precipitation. The entire procedure can be completed in 60 minutes. The purified total RNA is ready for RT, RT-PCR, real-time PCR, Northern blotting

The quality of Plant Total RNA Mini Kit is tested on a lot·to·lot basis. The kits are tested by isolation of total RNA from 2S mg of young leaves. Purified RNA is quantified and checked by agarose gel electrophoresis.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye