The Plant Total RNA Mini Kit provides a fast and simple method to isolate total RNA from plant tissue and cells. In this process the sample is first ground
in liquid nitrogen and filtrated by filter column to remove cell debris. In the presence of binding buffer and a chaotropic salt the total RNA in the lysate
binds to the glass fiber matrix in the spin column. The optional DNase treatments can remove DNA residues and contaminants are washed out with
an ethanol based wash buffer. Finally, the purified total RNA is eluted by RNase - free water. The protocol does not require phenol extraction and
Ethanol precipitation. The entire procedure can be completed in 60 minutes. The purified total RNA is ready for RT, RT-PCR, real-time PCR, Northern
blotting