The Total RNA Extraction Kit Mini (Tissue) is specially
designed for purification of total RNA from a variety of animal tissues or
cells. The provided micropestle efficiently homogenizes tissue samples in a
microcentrifuge tube. The method uses detergents and a chaotropic salt to lyse
cell and inactivate RNase. Lysate is clarified with provided lysate filter
columns. RNA in the chaotropic salt solution is bound to the glass fiber matrix
of the RB column. Following washing off of the contaminants the purified RNA is
eluted by RNase-free water. Purified RNA is ready for RT-PCR, northern
blotting, primer extension and cDNA library construction.