(DNase I, Deoxyribonucleodepolymerase, Deoxyribonucleate
5'-oligo-nucleotide hydrolase, Desoxyribonuclease, Nuklease, Ribonuklease, )
EC 3.1.21.1 • Mr 31 000 • CAS [9003-98-9]
Deoxyribonuclease I, or DNase I
- is used to nick DNA
as a first step to incorporate labelled bases into DNA
- is suitable for
DNase footprinting
- improves yield in
primary cell isolation by removing extracellular DNA
- reduces viscosity
in protein samples by removal of DNA contamination
DNase I is an endonuclease that cleaves phosphodiester bonds adjacent to
pyrimidines to produce polynucleotides with terminal 5′-phosphates. It
preferentially cleaves double-stranded DNA and, in the presence of Mg2+ ,
single-stranded DNA.
Unit definition: 1 Kunitz unit catalyses an increase in absorbance
of 0.001 at 260 nm per minute at 25 °C, pH 5.0, when exposed to highly
polymerised calf thymus DNA (1).
Activity: min. 3000 Kunitz units/mg lyophilisate
