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  2. SERVA Ribonuclease A from bovine pancreas, 50mg.

SERVA Ribonuclease A from bovine pancreas, 50mg.

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MT-CM-SV3438801
lyophil., DNase free


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(Pancreatic RNase, ribonuklease, ribonucleate 3-pyrimidino-oligonucleotidohydrolase)

EC 3.1.27.5 • Mr ca. 13 700 • CAS [9001-99-4]


Chromatographically homogeneous preparation of Ribonuclease A (RNase A), free of DNase, protease, and salt. A major application for RNase A is the removal of RNA from preparations of plasmid DNA. It is also used in RNA sequence analysis, protection assays and removal of RNA contained in protein samples. RNase A is an endonuclease which specifically attacks pyrimidine sites (Py/pN) at the 3'-phosphate group. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts. The enzyme is inhibited by heavy metal ions and competitively by DNA.

Unit definition: 1 unit is that amount of activity which is capable of causing within 1 minute a decrease in absorbance at 300 nm equivalent to the maximum possible change in a 0.05 % solution of yeast RNA at 25 °C, pH 5.0.

 

  1. Kunitz, M. (1946) J. Biol. Chem. 164, 563-8
  2. Krupp, G. & Gross, H.J. (1979) Nucl. Acids Res. 6, 3481-90
  3. Levy, C.C. & Kaepetzky, T.P. (1980) J. Biol. Chem. 255, 2153-9
  4. Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (5.81)
  5. Ed. Ausubel et al. (1994) Current Protocols in Molecular Biology, Massachusetts General Hospital & Harvard Medical School (3.13.1, 5.5.2, 4.7.3, 7.3.8)

DANGER


Hazard Statements

H334

Precautions

P261 - P285

Reaction

P304 +P341 - P342 +P311


EINECS: 232-646-6 • WGK: 1 • HS: 35079090

Storage Temperature: +2 °C to +8 °C


(X-Gal)

C14H15BrClNO6 • Mr 408.6 • CAS [7240-90-6]


X-Gal is a well known histochemical substrate used to detect the β-galactosidase enzyme (1, 2, 3, 4, 9, 10). Identification of lacf-colonies (8). X-gal is used to distinguish recombinant plasmids from parental vectors in cloning experiments using vectors containing the lacZ or lacZ [DMvP1](-)peptide gene (2, 3, 5). Upon hydrolysis, X-Gal yields a localized, insoluble blue precipitate making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. X-Gal has been used for the detection of coliforms (E. coli) in municipal water supplies (6) and food products (7). X-Gal is often used in conjunction with IPTG (Isopropyl-β-D-thiogalactopyranoside, cat. no. 26600) which binds and inhibits the lac repressor thus inducing β-galactosidase activity.

Stock solution: 20 mg/ml in DMF. Store in aliquots at -20 °C in the dark. For detection of transformants, use in final concentration of 40 µg/ml.


Purity (HPLC)

min. 99.0 %