biological source:
rabbit
Quality Level: 200
Conjugate: unconjugated
antibody form: whole
antiserum
antibody product type:
secondary antibodies
clone: polyclonal
contains: 15 mM
sodium azide
technique(s):
indirect ELISA: 1:150,000
quantitative precipitin assay: 4.0 mg/mL
shipped in: dry
ice
storage temp.: −20°C
target post-translational modification: unmodified
Application
Anti-Bovine IgG (whole molecule) antibody produced in rabbit
has been used as a standard to determine protein content by Bradford method
using Bio-Rad protein assay dye reagent. It has also been used in several
assays to develop a particle concentration fluorescence immunoassay for the
quantitative determination of IgG in bovine milk.
Anti-Bovine IgG (whole molecule) antibody produced in rabbit
has been used for detecting IgG antibodies in multichannel monolithic quartz
crystal microbalance (MQCM).
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum
immunoglobulins of the immune system. It is secreted by B cells and is found in
blood and extracellular fluids and provides protection from infections caused
by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through
the placenta that is vital for immune defense of the neonate against
infections.
Immunoglobulins G (IgGi, IgGa) of bovine milk is capable of
protecting the gut mucosa against microorganisms.
The antiserum is determined to be immunospecific for bovine
IgG.
Physical form
Rabbit Anti-Bovine IgG is supplied as a liquid containing
0.1% sodium azide (see MSDS) as a preservative
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For
extended storage, the solution may be frozen in working aliquots. Repeated
freezing and thawing is not recommended. Storage in "frost-free"
freezers is not recommended. If slight turbidity occurs upon prolonged storage,
clarify the solution by centrifugation before use.
treated to remove lipoproteins
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
