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TOYOBO Hot Start TTx (RNA) Kit

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MT-MC-HSTTX-111

 
 
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  • Highly efficient one-enzyme system and 1-step qRT-PCR
    TTx DNA Polymerase has high reverse transcriptase activity, allowing efficient qRT-PCR even with small quantities of template. This reagent can also be used to detect DNA.
  • Detectability with high-speed cycles
    Taking advantage of the high amplification efficiency, TTx DNA Polymerase allows efficient amplification even with high-speed cycles.
  • Reverse transcription is possible at high temperatures
    The reverse-transcription reaction is possible at 60℃, which makes this reagent suitable for amplifying targets that are GC-rich or that easily adopt higher-order structures.

    This is a one-step qRT-PCR reagent using our unique enzyme TTx DNA Polymerase. TTx DNA Polymerase has reverse transcriptase activity, and this activity is strongly promoted in the presence of Mn2+. This activity can be used to perform reverse-transcription reactions and PCR with the same enzyme. Because TTx DNA Polymerase has reverse transcriptase activity, it can amplify with high efficiency from both DNA and RNA. TTx DNA Polymerase also has 5'→3' exonuclease activity, so it can be used for real-time PCR, for example using TaqMan® probes. This reagent contains a mixture of neutralizing antibodies, allowing for highly specific Hot Start PCR.


  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye


  •  QPS-101T
    • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
    • 50x ROX reference dye    50 µl × 1
  •  
  • QPS-101
    • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
    • 50x ROX reference dye    250 µl × 1
  •  
  • QPS-101X5
    • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
    • 50x ROX reference dye    250 µl × 5




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye