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TOYOBO KOD FX Neo

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MT-MC-KFX-201

High Efficient & success rate DNA polymerase  
 
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    • KOD -Plus- Neo exhibits 80-fold greater PCR fidelity than Taq DNA polymerase.
    • "Elongation enhancer" enables greater amplification efficiency and elongation capability (up to 24 kb from human genomic DNA) compared to conventional PCR.
    • Requires only 30 sec/kb for the PCR extension step.
    • 2-step cycle conditions can be used for amplification using ≥ 20 mer primers (melting temperatures, Tm >63ºC).

    KOD FX Neo is based on the DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1(1) (2). KOD FX Neo provides greater efficiency and elongation capabilities than conventional PCR enzymes. In particular, KOD FX Neo shows greater amplification success from crude specimens.
    KOD FX Neo is the improved version of the previous KOD FX (Code No. KFX-101). This product contains a unique "elongation enhancer" that suppresses the "plateau effect", enabling greater elongation rates and capabilities.
    The KOD FX Neo enzyme solution contains two types of anti-KOD DNA polymerase antibodies that inhibit the polymerase and 3'→5' exonuclease activities, thus allowing for Hot Start PCR(3). KOD FX Neo generates blunt-end PCR products because of its 3'→5' exonuclease (proof-reading) activity.



  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye


  •  QPS-101T
    • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
    • 50x ROX reference dye    50 µl × 1
  •  
  • QPS-101
    • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
    • 50x ROX reference dye    250 µl × 1
  •  
  • QPS-101X5
    • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
    • 50x ROX reference dye    250 µl × 5




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye