Effective amplification from templates containing uracils (U) and primers containing uracil (U) or Inosine (I) can be used
High fidelity
Applicable for amplification from crude samples
Highly efficiency
KOD -Multi & Epi-™ is a high-fidelity PCR enzyme based on genetically modified KOD DNA polymerase(UKOD). This modified enzyme enables amplification from templates containing uracils (U) or using primers containing inosines (I) and uracils (U). Furthermore, addition of the Elongation Accelerator significantly reduces amplification bias during PCR. KOD -Multi & Epi-™ can be applied to various purposes such as a) multiplex PCR, b) bisulfite PCR in epigenetics research, and c) metagenomics research. This enzyme is also applicable for the preparation of DNA fragments for next-generation sequencing and capillary sequencing via cloning because the enzyme exhibits about 11-fold higher PCR fidelity. KOD -Multi & Epi-™ contains two types of anti-KOD DNA polymerase antibodies that inhibit the polymerase and its 3ʹ→5ʹ exonuclease activity, thus allowing for Hot Start PCR. Furthermore, KOD -Multi & Epi-™ generates blunt-end PCR products because of its 3ʹ→5ʹ exonuclease (proof-reading) activity.
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
QPS-101T
THUNDERBIRD® Probe qPCR Mix 1 ml × 1
50x ROX reference dye 50 µl × 1
QPS-101
THUNDERBIRD® Probe qPCR Mix 1.67 ml × 3
50x ROX reference dye 250 µl × 1
QPS-101X5
THUNDERBIRD® Probe qPCR Mix (1.67 ml × 3) x 5
50x ROX reference dye 250 µl × 5
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
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