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TOYOBO KOD One™ PCR Master Mix

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Linear Formula:  H2SO4

CAS Number:  7664-93-9

Molecular Weight:  98.08

Beilstein:  2037554

MDL number:  MFCD00064589

UNSPSC Code:  12352301NACRES:NA.21


- Fast

KOD OneTM series can amplify the targets using the following very short conditions:

  • ≤1 kb:　1 sec
  • 1~ 10 kb:　5 sec/ kb
  • 10 kb~:　10 sec/ kb

The cycling conditions can be set flexibly when various targets having different sizes are amplified.

- Easy to Use

KOD OneTM series contains all reaction components except primers and templates and provide high reproducibility by reducing operations. In addition, KOD OneTM PCR Master Mix -Blue- includes a loading dye (BPB) to allow direct loading onto agarose gels.

- High Fidelity

KOD OneTM series exhibits approximately 80-fold higher fidelity than Taq DNA polymerase. These mixes can be used for various purposes where this would be an advantage (e.g., in the preparation of long target amplicons for sequencing).

- High Efficiency

KOD OneTM series is effective for amplification from crude samples (e.g., biological samples, foodstuffs, soil extract, etc.). Various samples or lysates can be used directly as templates.

- Primers or Templates Containing Inosines (dI) or Uracils (dU) Can Be Used

KOD OneTM series can use primers or templates containing inosines (dI) or uracils (dU), whereas conventional high-fidelity PCR enzymes cannot.


  • KOD One™ PCR master Mix and KOD One™ PCR Master Mix -Blue- are 2 x PCR master mixes based on genetically modified KOD DNA polymerase (UKOD). KOD One™ series enables fast PCR, which has an extension time of 5 sec/ kb by applying UKOD and a new Elongation Accelerator. In addition, these master mixes provide greater efficiency and elongation capabilities than conventional PCR enzymes. In particular, these show greater amplification success from crude specimens. Furthermore, these master mixes can be applied to amplify from templates containing uracils (dU) or using primers containing inosines (dI) and uracils (dU).
  • KOD One™ series contains two types of anti-KOD DNA polymerase antibodies that inhibit the polymerase and 3’→5’ exonuclease activities, thus allowing for Hot Start PCR. These master mixes generate blunt-end PCR products because of 3’ → 5’ exonuclease (proof-reading) activity of KOD DNA polymerase.