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TOYOBO KOD -Plus- Neo

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MT-MC-KOD-401

High Fidelity & efficient & fast DNA polymerase  
 
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  • KOD -Plus- Neo exhibits 80-fold greater PCR fidelity than Taq DNA polymerase.
  • "Elongation enhancer" enables greater amplification efficiency and elongation capability (up to 24 kb from human genomic DNA) compared to conventional PCR.
  • Requires only 30 sec/kb for the PCR extension step.
  • 2-step cycle conditions can be used for amplification using ≥ 20 mer primers (melting temperatures, Tm >63ºC).

    KOD -Plus- Neo is based on a DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1(1) (2). This polymerase exhibits excellent PCR fidelity because of its efficient 3'→5' exonuclease activity (proof reading activity). This product contains a unique "elongation enhancer" that suppresses the "plateau effect" produced by conventional PCR. Therefore, this reagent exhibits greater amplification efficiency and elongation capability compared to the previous version of KOD -Plus- (Code No. KOD-201).

    Moreover, this enzyme requires only 30 sec/kb for the PCR extension step. This facilitates the long range PCR.
    This enzyme contains two anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR(3). This polymerase generates blunt-end PCR products due to 3'→5' exonuclease (proof-reading) activity.

  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye


  •  QPS-101T
    • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
    • 50x ROX reference dye    50 µl × 1
  •  
  • QPS-101
    • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
    • 50x ROX reference dye    250 µl × 1
  •  
  • QPS-101X5
    • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
    • 50x ROX reference dye    250 µl × 5




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye