TOYOBO THUNDERBIRD™ Probe One-step qRT-PCR Kit

• THUNDERBIRD® Probe One-step qRT-PCR Kit is a one-step real-time reverse-transcription polymerase chain reaction (RT-PCR) kit that uses the highly efficient reverse transcriptase “ReverTra Ace®” and Tth DNA polymerase as a PCR enzyme. This product can be used mainly in TaqMan® probe assays. The one-step system is suitable for high-throughput analysis because of its simple reaction setup. In addition, this system can reduce the risk of cross-contamination. The combination of the two enzymes and optimized buffer system enable the effective detection and quantification of a small amount of RNA. This kit can also detect various kinds of RNA with different sequences because it is tolerant of target sequence diversity.
  

This kit includes the following components for 250 reactions, with 20 µL per reaction. All reagents should be stored at −20°C.

2x Reaction Buff*                2x 1.25 ml 

DNA Polymerase                       125 µl 

RT Enzyme Mix                         125 µl 

50x ROX Reference dye           100 µl

RNase free water                2x 1.25 ml *12× Reaction Buffer contains essential components for the reaction (buffer, salts, dATP, dCTP, dGTP, and dUTP).

Uracil-N-glycosylase is not supplied with this kit. 

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye