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  2. TOYOBO Uracil-DNA Glycosylase (UNG), Heat-labile

TOYOBO Uracil-DNA Glycosylase (UNG), Heat-labile

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MT-MC-UNG-101

 
 
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  • Useful in preventing PCR carry-over and false positives
  • Because of its high thermal sensitivity, it can be used not only for PCR but also for RT-PCR (RT-reaction should be performed at 42°C or higher)


    Uracil-DNA Glycosylase (UNG) hydrolyzes the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an apyrimidinic site in DNA. Since DNA with an apyrimidinic site is degraded by heat treatment, UNG can be used in combination with dUTP to prevent carry-over of amplified products derived from PCR.
    This enzyme hydrolyzes uracil from both single- and double-stranded DNA containing dU, but it not from RNA. It can also be used to prevent carryover of RT-PCR.
    We also have a glycerol-free version that is suitable for lyophilization.

     

  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye


  •  QPS-101T
    • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
    • 50x ROX reference dye    50 µl × 1
  •  
  • QPS-101
    • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
    • 50x ROX reference dye    250 µl × 1
  •  
  • QPS-101X5
    • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
    • 50x ROX reference dye    250 µl × 5




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye